منابع مشابه
Extracellular Maltase of Bacillus brevis.
Bacillus brevis NRRL B-4389 produced extracellular maltase (alpha-glucosidase; EC 3.2.1.20) only in the presence of short alpha-1,4-glucosidic polymers, such as maltose and maltotriose. An optimum medium was developed; it contained 2.5% maltose, 0.5% nonfat dry milk, 0.4% yeast extract, and 0.01% CaCl(2). The enzyme was produced extracellularly during the logarithmic phase of growth; no cell-bo...
متن کاملA protein disulfide isomerase gene fusion expression system that increases the extracellular productivity of Bacillus brevis.
We have developed a versatile Bacillus brevis expression and secretion system based on the use of fungal protein disulfide isomerase (PDI) as a gene fusion partner. Fusion with PDI increased the extracellular production of heterologous proteins (light chain of immunoglobulin G, 8-fold; geranylgeranyl pyrophosphate synthase, 12-fold). Linkage to PDI prevented the aggregation of the secreted prot...
متن کاملCharacterization of an extracellular protease inhibitor of Bacillus brevis HPD31 and nucleotide sequence of the corresponding gene.
A novel proteinaceous protease inhibitor was isolated from the culture supernatant of Bacillus brevis HPD31. The protease inhibitor of B. brevis (designated BbrPI) was produced extracellularly in multiple forms having at least three different molecular weights. One of them, BbrPI-a, was purified to near homogeneity and only showed inhibitory activity toward serine proteases, such as trypsin, ch...
متن کاملFormation of Tyrothricin in Submerged Cultures of Bacillus Brevis.
Since the isolation of tyrothricin from cultures of Bacillus brevis (Dubos, 1939), numerous papers have appeared dealing with the chemical and biological properties of this bactericidal agent and its components, gramicidin and tyrocidine (Hotchkiss and Dubos, 1940; Dubos and Hotchkiss, 1941; Tishler, Stokes, Trenner and Conn, 1941; Christensen, Edwards and Piersma, 1941; Lipmann, Hotchkiss and ...
متن کاملThe cellular D-amino acids of Bacillus brevis.
In a previous paper (1) evidence was presented that acid hydrolysates of the cellular material of BaciZZus brevis (ATCC 10068) contained an unusually high content of amino acids of the D configuration, amounting to over 10 per cent of the total amino acids or 5 per cent of the dry weight of the cells. The n-amino acids were shown to exist in the intact cells, and not to result from racemization...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1979
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.37.6.1096-1102.1979